Product Number: P9730 Storage Temperature: -20 °C CAS Number: 9001-77-8 EC Number: 3.1.3.2 Synonyms: Apase; Orthophosphoric-monoester phosphohydrolase (acid optimum) Product Description Acid phosphatases (APase) are a family of enzymes that non-specifically catalyze the hydrolysis of monoesters and anhydrides of phosphoric acid to produce inorganic phosphate at an optimum pH of 4 to 7 by the following reaction: R-PO4 + H2O→R-OH + HOPO3 2+ Their function in the production, transport, and recycling of phosphate is critical for the metabolic and energy transduction processes of the cell. As a group, APases may be as important as kinases in regulatory processes. Plant APases have been localized in the cytosol, vacuoles,and cell walls. One key role is phosphate acquisition to mobilize organic phosphates in the soil.Phosphate starvation also induces APase generation. Potato tuber APase is a monomeric glycoprotein. The carbohydrate component makes up 16.6% of the molecular mass. Molecular mass: 69 kDa Carbohydrate residues (%):Mannose 5.0, Glucosamine 3.6, Rhamnose 3.4, Glucose 2.5, Galactose 1.5 pH Optimum: 5.0–5.3 pH Range: 4–7 Temperature optimum: 37 °C Substrates:α-glyceryophosphate; ATP, fructose-6-phosphate, fructose-6-phosphate, inorganic pyrophosphate, glucose-6-phosphate, p-nitrophenyl phosphate KM(mM): p-nitrophenyl phosphate 1.25, inorganic pyrophosphate 40.0 Activators:Cu2+, Mg2+, Mn2+, Zn2+, Hg2+(below 0.4 mM) Inhibitors:Al3+, Hg2+(above 0.4 mM), MoO4 2–, Zn2+, urea This product is partially purified from potato tubers and is supplied as a lyophilized powder. Specific activity: 0.5–3.0 units/mg solid Unit definition: One unit will hydrolyze 1.0 μmole of p-nitrophenyl phosphate per minute at pH 4.8 at 37 °C. APase is assayed spectrophotometrically in a 1.1 ml reaction mixture containing 41 mM citrate buffer, pH 4.8 at 37 °C, 6.9 mM p-nitrophenyl phosphate, and 0.015–0.025 unit APase. Preparation Instructions APase is soluble in cold water (0.15–0.25 unit/ml). Prepare solution immediay before use. Storage/Stability Store the product at –20 °C. When stored at –20 °C, the enzyme retains activity for at least one year. APase can lose half its activity after 30 minutes at 60 °C and 100% of its activity after two hours at 70 °C. Precautions and Disclaimer
